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Bethyl anti xpc
Anti Xpc, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+xpc/XPC+Antibody/pmc12644609-254-26-31
Average 93 stars, based on 38 article reviews
anti xpc - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

other:

Article Title: Proteomic discovery of DEK and NUMA1 as new players in UV-induced DNA damage repair mechanisms
Article Snippet: The following antibodies were utilized in this study: anti-α-TUBULIN (T9026, Sigma-Aldrich); anti-DEK (ab166624, Abcam); anti-NUMA1 (ab97585, Abcam; sc-365532, Santa Cruz); anti-CPD (CAC-NM-DND-002, Cosmobio); anti-(6-4)PPs (CAC-NM-DND-001, Cosmobio); anti-XPC (sc-74411, Santa Cruz; A301-121A, Bethyl Laboratories); anti-XPB (PA5-22254, Thermo Fisher Scientific); anti-XPD (ab54676, Abcam); anti-p62 (sc-48431, Santa Cruz); anti-XPA (sc-853 and sc-28353, Santa Cruz); anti-XPF (NBP2-58407, Novus Biologicals); anti-XPG (NB100-74611, Novus Biologicals; A301-484A, Bethyl Laboratories); anti-PCNA (ab18197, Abcam); anti-RFC1 (NBP2-54960, Novus Biologicals); anti-APE1 (ab189474, Abcam); anti-KU80 ( MA512933 , Invitrogen); anti-Biotin (sc-57636, Santa Cruz); anti-Histone H3 (07-690, Merck Millipore); Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 488 (A11008, Thermo Fisher Scientific); Cy3-Goat Anti-Mouse IgG (115-65-146, Jackson Immunoresearch); Horseradish peroxidase (HRP)-conjugated Goat anti-rabbit IgG antibody (ADI-SAB-300-J, Enzo); HRP-conjugated Goat anti-mouse IgG antibody (ADI-SAB-100-J, Enzo).

Incubation:

Article Title: SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response
Article Snippet: Then, the cells were permeabilized with 0.2% Triton X-100/PBS for 5 min. After washing steps, cells were blocked with 5% BSA (Sigma-Aldrich, St. Louis, MO, USA) in PBST (0.1% Tween 20 (Sigma-Aldrich, St. Louis, MO, USA) in PBS), supplemented with GAR-HPR (Dako, P0448, Santa Clara, CA, USA) and RAM-HPR (Dako, P0260, Santa Clara, CA, USA) in 1:2000 dilution for 20 min. .. Cells were washed with PBST, then incubated with primary antibodies diluted in 1% BSA/PBST: anti-PCNA (Santa Cruz, sc-7907, Santa Cruz, CA, USA) in 1:200 dilution, anti-RNAPII CTD 4H8 (Santa Cruz, sc-47701, Santa Cruz, CA, USA) in 1:100 dilution, and anti-XPC (Bethyl Laboratories, A301–122A, Montgomery, TX, USA) in 1:1000 dilution, which was kindly provided by Frederic Coin. .. After washing steps, fluorophore labeled secondary antibodies were used, like GAR Alexa 550 (Thermo Fisher Scientific, A21429, Waltham, MA, USA) and GAM Alexa 488 (Thermo Fisher Scientific, A11029, Waltham, MA, USA).

Western Blot:

Article Title: RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling
Article Snippet: .. The following antibodies were used for Western blotting: anti-RNF8 (B-2) (#sc-271462, 1:5000), anti-GFP (#sc-9996, 1:3000), anti-Ub (A-5) (#sc-166553, 1:1000), anti-CDC25A (F-6) (#sc-7389, 1:1000), anti-p53 (#sc-126, 1:1000) and anti-HSP90 (H-114) (#sc-7479, 1:2000) from Santa Cruz Biotechnology; anti-phospho H2AX S139 (#ab11174, 1:20000), anti-H2AX (#ab11175, 1:10 000) and anti-TOPBP1 (#ab2402, 1:5000) from Abcam; anti-phospho CHK1 S345 (#2348, 1:2500), anti-CHK1 (#sc-8408, 1:3000) and anti-MYC (#2276, 1:2000) from Cell Signaling Technology; anti-FLAG (#F3165, 1:10 000), anti-biotin (#B7653, 1:10 000) and anti-β-actin (#A2228, 1:10000) from Sigma; anti-NONO (#611279, 1:10000) from BD Transduction Laboratories; anti-T7 (#69522, 1:10000) from Novagen; anti-HA (#clone 12CA5, 1:1000) from Roche; anti-ATRIP (#A300-095A, 1:2000), anti-XPC (#A301-122A, 1:2000) and anti-SFPQ (#A301-320A, 1:2000) from Bethyl Laboratories, Inc.; anti-K48-specific ubiquitin (#clone Apu2.07, 1:1000) and anti-K63-specific ubiquitin (#clone Apu3.A8, 1:1000) from Genentech Inc. ..

Ubiquitin Proteomics:

Article Title: RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling
Article Snippet: .. The following antibodies were used for Western blotting: anti-RNF8 (B-2) (#sc-271462, 1:5000), anti-GFP (#sc-9996, 1:3000), anti-Ub (A-5) (#sc-166553, 1:1000), anti-CDC25A (F-6) (#sc-7389, 1:1000), anti-p53 (#sc-126, 1:1000) and anti-HSP90 (H-114) (#sc-7479, 1:2000) from Santa Cruz Biotechnology; anti-phospho H2AX S139 (#ab11174, 1:20000), anti-H2AX (#ab11175, 1:10 000) and anti-TOPBP1 (#ab2402, 1:5000) from Abcam; anti-phospho CHK1 S345 (#2348, 1:2500), anti-CHK1 (#sc-8408, 1:3000) and anti-MYC (#2276, 1:2000) from Cell Signaling Technology; anti-FLAG (#F3165, 1:10 000), anti-biotin (#B7653, 1:10 000) and anti-β-actin (#A2228, 1:10000) from Sigma; anti-NONO (#611279, 1:10000) from BD Transduction Laboratories; anti-T7 (#69522, 1:10000) from Novagen; anti-HA (#clone 12CA5, 1:1000) from Roche; anti-ATRIP (#A300-095A, 1:2000), anti-XPC (#A301-122A, 1:2000) and anti-SFPQ (#A301-320A, 1:2000) from Bethyl Laboratories, Inc.; anti-K48-specific ubiquitin (#clone Apu2.07, 1:1000) and anti-K63-specific ubiquitin (#clone Apu3.A8, 1:1000) from Genentech Inc. ..



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A , B CCK-8 assay to detect the inhibitory effect of NRG1 on cell death induced by Erastin, Nigericin, Hydroxyurea, and Wogonoside in BC cells ( n = 3). C Transmission electron microscopy to observe mitochondrial damage in MDA-MB-231 cells. Scale bar = 2 μm (Zoom in = 1 μm). D , E Western blot to detect the effect of exogenous NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). F , G Western blot to observe the effect of CAF-derived NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). Data are expressed as the mean ± S.D. for all panels: ns = no significance, # P < 0.05, ** , ## P < 0.01, ***,### P < 0.001.

Journal: Cell Death Discovery

Article Title: NRG1/PDGFC loop between fibroblasts and cancer cells drives paclitaxel resistance via ferroptosis suppression in breast cancer

doi: 10.1038/s41420-025-02785-2

Figure Lengend Snippet: A , B CCK-8 assay to detect the inhibitory effect of NRG1 on cell death induced by Erastin, Nigericin, Hydroxyurea, and Wogonoside in BC cells ( n = 3). C Transmission electron microscopy to observe mitochondrial damage in MDA-MB-231 cells. Scale bar = 2 μm (Zoom in = 1 μm). D , E Western blot to detect the effect of exogenous NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). F , G Western blot to observe the effect of CAF-derived NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). Data are expressed as the mean ± S.D. for all panels: ns = no significance, # P < 0.05, ** , ## P < 0.01, ***,### P < 0.001.

Article Snippet: PDGFC recombinant protein (HY-P7279), Paclitaxel (PTX, HY-B0015), ARQ 751 trihydrochloride (HY-137458A), Erastin (HY-15763), Nigericin (HY-127019), Hydroxyurea (HY-B0313), Wogonoside (HY-N0399), and PDGFRα kinase inhibitor 1 (HY-111507) were purchased from MedChemExpress (USA), while NRG1 recombinant protein was obtained from Cell Signaling Technology (26941).

Techniques: CCK-8 Assay, Transmission Assay, Electron Microscopy, Western Blot, Derivative Assay